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phic31o coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc phic31o coding sequence
    Phic31o Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phic31o+coding+sequence/Lamp1-CFP+(Plasmid+%231815)/pm41102823-99-8-15
    Average 88 stars, based on 2 article reviews
    phic31o coding sequence - by Bioz Stars, 2026-09
    88/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse.
    Article Snippet: The maximum intensity projection image of a confocal z-stack was obtained using Fiji/ImageJ (National Institutes of Health, Bethesda, MD, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5’ BamHI and 3’ EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Briefly, digoxigenin-labeled phiC31o, Opn3, and Chml antisense RNA probes were hybridized to sections, NBT/BCIP (Roche, Basel, Switzerland) was used for color development, and nuclear fast red (Sigma Aldrich, St. Louis, MO, USA) was used for counterstaining.

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse
    Article Snippet: To generate the Opn3 probe, a plasmid with the same sequence as the Opn3 probe used in the Allen Brain Atlas (Opn3-RP 050331_04_B02) was synthesized at GenScript Biotech (Piscataway, NJ, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5' BamHI and 3' EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). ..

    Sequencing:

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse.
    Article Snippet: The maximum intensity projection image of a confocal z-stack was obtained using Fiji/ImageJ (National Institutes of Health, Bethesda, MD, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5’ BamHI and 3’ EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Briefly, digoxigenin-labeled phiC31o, Opn3, and Chml antisense RNA probes were hybridized to sections, NBT/BCIP (Roche, Basel, Switzerland) was used for color development, and nuclear fast red (Sigma Aldrich, St. Louis, MO, USA) was used for counterstaining.

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse
    Article Snippet: To generate the Opn3 probe, a plasmid with the same sequence as the Opn3 probe used in the Allen Brain Atlas (Opn3-RP 050331_04_B02) was synthesized at GenScript Biotech (Piscataway, NJ, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5' BamHI and 3' EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). ..

    Plasmid Preparation:

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse.
    Article Snippet: The maximum intensity projection image of a confocal z-stack was obtained using Fiji/ImageJ (National Institutes of Health, Bethesda, MD, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5’ BamHI and 3’ EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Briefly, digoxigenin-labeled phiC31o, Opn3, and Chml antisense RNA probes were hybridized to sections, NBT/BCIP (Roche, Basel, Switzerland) was used for color development, and nuclear fast red (Sigma Aldrich, St. Louis, MO, USA) was used for counterstaining.

    Article Title: Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse
    Article Snippet: To generate the Opn3 probe, a plasmid with the same sequence as the Opn3 probe used in the Allen Brain Atlas (Opn3-RP 050331_04_B02) was synthesized at GenScript Biotech (Piscataway, NJ, USA). .. To make the phiC31o probe, we cloned the phiC31o coding sequence (1815 bp from pPGKPhiC31obpA (addgene plasmid number 13795) into 5' BamHI and 3' EcoRI of pBlueScript II SK ( −) (Agilent Technologies, Inc., Santa Clara, CA, USA). ..



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    phic31o coding sequence  (Addgene inc)
    88
    Addgene inc phic31o coding sequence
    Phic31o Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phic31o+coding+sequence/Lamp1-CFP+(Plasmid+%231815)/pm41102823-99-8-15
    Average 88 stars, based on 1 article reviews
    phic31o coding sequence - by Bioz Stars, 2026-09
    88/100 stars
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